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In functional assays, IC50 is the concentration that reduces a biological response, e.g., transcription of mRNA or protein expression, by 50% of the biological response that is achieved without the therapeutic molecule. IC50 is the median inhibitory concentration of a therapeutic molecule. Non-limiting examples of the therapeutic molecules include oligomers, one or more nucleotides, one or more nucleosides, one or more amino acids, polynucleotides, peptides, proteins, polypeptides, or small molecule compounds that are naturally occurring, modified, recombinantly produced, or chemically synthesized